480 Individual Control DNA Samples Available For Immediate Use
Introduction
The DNA in the panels has been extracted from lymphoblastoid cell lines derived by EBV transformation of peripheral blood lymphocytes from single donor blood samples. The donors are ethnically matched as UK Caucasian and characterised by gender and age at venesection. Informed consent has been obtained for their use in research. A series of 5 panels (HRC-1 to 5) are NOW available, each comprised of 96 individuals and all are sourced from cell lines matching the above criteria.
The MRC Geneservice (now known as Geneservice) have had excellent results with the first panel, HRC-1 (96 array), as part of their SNP analysis programme using the TaqMan assay. The panels provide an ideal control population for the quality control of routine assays in addition to numerous uses in genetic research. DNA from this resource has already been used by other investigators such as:
| Researcher /Organisation | Disorder | | Professor John Todd | Type I Diabetes | | Professor Andrew Hattersley | Type II Diabetes | | Professor Jane Worthington | Rheumatoid Arthritis | | Sanger Institute | Various Cancer Research Programmes | | National Institute of Health | Mood and Anxiety |
Key Features - No problem of batch-to-batch variation as the DNA is manufactured from immortalised cell lines held as cryopreserved banks Authentication - batch-to-batch consistency is confirmed using STR PCR at the MRC HGMP UK Resource Centre, Cambridge, U.K High Purity and High Molecular Weight - Rigorous quality control procedures are followed to ensure that each batch meets the highest standard Reproducibility- The use of EBV-immortalised lymphoblastoid cell lines ensures an infinite supply of the standardised DNA that represents the genome of the original blood donor Individuality - Each DNA panel contains 96 individual samples, each representing a different donor subject Consent - All donors have given written, informed consent for their blood to be used for research purposes Immediately Available from Stock- Ideal for most standard genetic research applications and avoids error in preparing controls Cost Effective -the 10m g format provides enough purified HRC DNA for thousands of PCR assays Delivery Format & Quantity
ECACC HRC DNA panels (HRC 1-5) are available immediately from stock. All five DNA panels (HRC 1-5) are presented in the standard tube format of 96 x 10mg or 96 x 50mg amounts at a concentration of 0.2mg per m l. A convenient 96 well array format is also available for two of the panels (HRC-1 and HRC-2). The arrays are sealed with foil which is pierceable by metal tips or easily removed. A replacement foil is included should you need to store the array for future use. The HRC 96 well array panels are supplied at a concentration ready for immediate use in PCR reactions without dilution.
Application
- Polymorphism analysis
- Reference Standards
- Standards for diagnostic kits
- Population studies
- Investigating genetic diseases
- Gender studies
- Genomic DNA library construction
- Southern Blotting
- PCR
- Profiling studies in gene expression
And Much More!
Do you need a larger Control Population ?
ECACC has realised that larger numbers of individual genomic DNA samples are often required, sometimes in the thousands. To satisfy such needs, ECACC can supply DNA extracted from lymphocytes that are recovered from the filters used to remove the white cells from donated blood (Patent Pending). Using this novel approach ECACC is able to provide large numbers of purified DNA samples. Because the purified DNA originates from a finite source, all orders will be satisfied on a contract basis.
Ordering Information | Product Name | Product No. | Package Size | Concentration | Price (£) | | HRC-1 DNA Panel | HRC1 | 96 x 10µg | 200 ng/µl | 1450 | | HRC-2 DNA Panel | HRC2 | 96 x 10µg | 200 ng/µl | 1450 | | HRC-3 DNA Panel | HRC3 | 96 x 10µg | 200 ng/µl | 1450 | | HRC-4 DNA Panel | HRC4 | 96 x 10 µg | 200 ng/µl | 1450 | | HRC-5 DNA Panel | HRC5 | 96 x 10µg | 200 ng/µl | 1450 | | HRC-1 DNA Panel | HRC1 | 96 x 400ng (array) | 8 ng/µl | 495 | | HRC-2 DNA Panel | HRC2 | 96 x 400ng (array) | 8 ng/µl | 495 |
How to Order
Complete the online order form to order our exclusive range of HRC DNA panels. For more information contact our technical support co-ordinator on Tel: + 44 1980 612684 or email:[email protected].
Distribution
ECACC HRC DNA panels are NOW all available directly through ECACC or by contacting Sigma-Aldrich USA / Europe and Dainippon Sumitomo Pharmaceutical Co. (Japan). Visit their website for more details.
Additional Information
DNA is provided as a solution in 20mM Tirs(pH 8.0) 1mM EDTA to preserve DNA quality. The DNA concentration is measured using the ds DNA Quantification kit (Molecular Probes, USA) on a Fluroskan Ascent from Labsystems (Type 374).
Future Details
Full details of each HRC panels including technical datasheets are available online or by contacting our Technical Support Co-ordinator.
Other Cell Line DNA
ECACC is able to provide genomic DNA from any of its cell lines. The genomic DNA provides available from our DNA resource includes: - Ethnic DNA - Do you require reference DNA from individuals of a particular ethinic origin? ECACC has the answer.
- Primate DNA - avaialble from a range of non-human primate species
- Human Genetic Disease DNA - DNA is available from thousands of individuals each diagnosed with a specific disorder such as diabetes
- HLA Genetic Disease DNA - ECACC offers DNA from over 300 reference cell lines for which HLA-typing data is available
Reference
Click to view a selection of publications including the use of ECACC HRC DNA.
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