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Human Random Control DNA Panels
Product Data Sheet

Manufactured by:

Product Name: HRC-5 (2µg)
Catalogue No: 06041305
Delivery State: HRC-5 DNA Panel (96 x 2µg)
Price: £200 per DNA Panel

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Intended Use
ECACC Human Random Control (HRC) DNA Panels are intended as an aid to determining the characteristics of DNA from apparently normal, randomly selected subjects as a basis for comparison with DNA from subjects that have been selected for particular phenotypic characters. The DNA arrays are designed for single use and can be used directly in automated gene analysis systems.

Description
HRC DNA is extracted from lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) transformation of peripheral blood lymphocytes from fresh, single donor blood samples. The donors are all UK Caucasian and are characterised by gender and age at venesection. All donors have given written, informed consent for their blood to be used for research purposes.

The DNA is extracted and purified using standard techniques that yield high molecular weight material, as verified by agarose gel electrophoresis. The composition of each panel is completely defined and standardised so that each product lot will be identical. The identity of each product lot is checked using STR DNA profiling. Consequently ECACC HRC DNA Panels can be used as Reference Standard material as an aid to routine quality control in the laboratory.

Sample details
The table below provides the individual sample designation.

  1 2 3 4 5 6 7 8 9 10 11 12
A C2078 C2090 C2110

C2111

C2098 C2126 C2141 C2145 C2150 C2153 C2155 C2173
B C2175 C2186 C2188 C2152

C2187

C2190 UKTS
8802
C2185 UKTS
8727
UKTS
8840
UKTS
8825
UKTS
8878
C UKTS
9115
UKTS
9213
SG
0004
SG
0005
SG
0010
SG
0002
SG
0015
SG
0018
SG
0022
SG
0008
UKTS
9050
UKTS
9082
D

SG
0020

SG
0038
SG
0024
SG
0041
SG
0034
SG
0040
SG
0026
SG
0030
UKTS
9142
UKTS
9175
UKTS
9242
SG
0053
E SG
0054
SG
0055
SG
0062
SG
0063
SG
0064
SG
0067
SG
0074
SG
0066
SG
0069
SG
0071
SG
0077
SG
0090
F SG
0094
SG
0095
SG
0097
SG
0100
SG
0101
SG
0102
SG
0099
SG
0105
SG
0081
SG
0060
SG
0086
SG
0084
G SG
0070
SG
0080
SG
0088
SG
0089
SG
0104
SG
0111
SG
0035
SG
0082
SG
0073
SG
0109
SG
0057
SG
0073
H SG
0059
SG
0112
SG
0072
SG
0046
SG
0052
SG
0114
SG
0115
SG
0117
SG
0118
SG
0120
SG
0003
SG
0107


Amount and concentration of DNA
The DNA concentration is measured using the ds DNA Quantitation kit (Molecular Probes, USA) on a Fluroskan Ascent from Labsystems (Type 374).

The DNA is dissolved in 10mM Tris (pH8) 1mM EDTA to preserve the quality of the DNA.

Total DNA per sample: Minimum of 2µg
Concentration: Target 100ng/µl Typical range +/- 10ng/µl
Recoverable volume: 20µl

Storage
ECACC DNA arrays are supplied frozen to preserve quality and also to facilitate handling. On receipt, panels should be stored at -80°C. The DNA array presentation is intended for single use although, with care, repeat use is possible. After thawing, panel can be stored at 2-8 °C for 6 months, provided precautions are taken to prevent fluid loss by evaporation.

Repeated freeze-thawing cycles can damage the DNA and should be avoided.

Use of DNA Panels
Remove the panel from its polythene bag / packaging and make sure this Data Sheet is retained safely. Do not remove the foil seal.

Place the panel on a level surface, top uppermost and allow to thaw. Centrifuge the plate to force all the fluid to the bottom of the well. Do not remove the foil seal until these precautions have been observed.

Important - If it is not possible to centrifuge the plates then the foil seal must be removed immediately after removal from freezer storage. (If the plate is allowed to thaw, liquid may condense on the underside of the foil seal.)

Alternatively the foil seal may be pierced using a sharp point to allow access to micropipette tips. This approach minimises the risk of cross contamination associated with the action of peeling off the foil seal.

Use a micropipette to withdraw the required amount of DNA solution from the array.

Warnings and Precautions
Avoid cross contamination between different samples comprising an array. Do not repeatedly use the same foil seal. Remove the seal with care. Use clean pipette tips for each specimen.

The master cell banks used to prepare the DNA extracts are taken from 'low risk' normal blood donors. However it is recommended that standard laboratory precautions for handling potentially infectious material should be employed.

Limitations of Use
ECACC DNA Panels are intended for research use only.

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