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Human Random Control DNA Panels
Product Data Sheet

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Product Name: HRC-4 (2µg)
Catalogue No: 06041304
Delivery State: HRC-4 DNA Panel (96 x 2µg)
Price: £200 per DNA Panel

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Intended Use
ECACC Human Random Control (HRC) DNA Panels are intended as an aid to determining the characteristics of DNA from apparently normal, randomly selected subjects as a basis for comparison with DNA from subjects that have been selected for particular phenotypic characters. The DNA arrays are designed for single use and can be used directly in automated gene analysis systems.

Description
HRC DNA is extracted from lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) transformation of peripheral blood lymphocytes from fresh, single donor blood samples. The donors are all UK Caucasian and are characterised by gender and age at venesection. All donors have given written, informed consent for their blood to be used for research purposes.

The DNA is extracted and purified using standard techniques that yield high molecular weight material, as verified by agarose gel electrophoresis. The composition of each panel is completely defined and standardised so that each product lot will be identical. The identity of each product lot is checked using STR DNA profiling. Consequently ECACC HRC DNA Panels can be used as Reference Standard material as an aid to routine quality control in the laboratory.

Sample details
The table below provides the individual sample designation.

  1 2 3 4 5 6 7 8 9 10 11 12
A C0963 C0971 C0976

C0981

C0983 C0988 C0995 C2012 C2013 C2018 C2020 C2021
B C2022 C2023 C2025 C2026

C2027

C2039 C2046 C2048 C2070 C2071 C2074 C2081
C C2085 C2089 C2091 C2093 C2102 C2103 C2108 C2109 C2113 C2114 C2116 C2118
D

C2119

C2120 C2121 C2122 C2125 C2127 C2130 C2132 C2135 C2139 C2143 C2144
E C2146 C2148 C2151 C2156 C2158 C2159 C2160 C2161 C2172 C2176 C2179 C2180
F C2182 C2183 C2184 UKTS
8049
UKTS
8670
UKTS
8754
C2191 UKTS
8695
UKTS
8791
UKTS
8792
UKTS
8796
UKTS
8797
G UKTS
8837
C2174 UKTS
8992
C2142 UKTS
8740
UKTS
8768
UKTS
9019
UKTS
8966
UKTS
9110
UKTS
9109
UKTS
8713
UKTS
9074
H UKTS
9914
C2072 UKTS
8989
UKTS
9002
UKTS
9051
UKTS
9003
UKTS
9020
UKTS
9025
UKTS
9046
UKTS
9201
UKTS
9225
UKTS
9260


Amount and concentration of DNA
The DNA concentration is measured using the ds DNA Quantitation kit (Molecular Probes, USA) on a Fluroskan Ascent from Labsystems (Type 374).

The DNA is dissolved in 10mM Tris (pH8) 1mM EDTA to preserve the quality of the DNA.

Total DNA per sample: Minimum of 2µg
Concentration: Target 100ng/µl Typical range +/- 10ng/µl
Recoverable volume: 20µl

Storage
ECACC DNA arrays are supplied frozen to preserve quality and also to facilitate handling. On receipt, panels should be stored at -80°C. The DNA array presentation is intended for single use although, with care, repeat use is possible. After thawing, panel can be stored at 2-8 °C for 6 months, provided precautions are taken to prevent fluid loss by evaporation.

Repeated freeze-thawing cycles can damage the DNA and should be avoided.

Use of DNA Panels
Remove the panel from its polythene bag / packaging and make sure this Data Sheet is retained safely. Do not remove the foil seal.

Place the panel on a level surface, top uppermost and allow to thaw. Centrifuge the plate to force all the fluid to the bottom of the well. Do not remove the foil seal until these precautions have been observed.

Important - If it is not possible to centrifuge the plates then the foil seal must be removed immediately after removal from freezer storage. (If the plate is allowed to thaw, liquid may condense on the underside of the foil seal.)

Alternatively the foil seal may be pierced using a sharp point to allow access to micropipette tips. This approach minimises the risk of cross contamination associated with the action of peeling off the foil seal.

Use a micropipette to withdraw the required amount of DNA solution from the array.

Warnings and Precautions
Avoid cross contamination between different samples comprising an array. Do not repeatedly use the same foil seal. Remove the seal with care. Use clean pipette tips for each specimen.

The master cell banks used to prepare the DNA extracts are taken from 'low risk' normal blood donors. However it is recommended that standard laboratory precautions for handling potentially infectious material should be employed.

Limitations of Use
ECACC DNA Panels are intended for research use only.

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