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Xhulhoven

 

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Joined: 14/3/2006
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Vero-Hektor  
On the short description of vero-hektor subcultures it is written that EDTA only must be used (no trypsin). We did not succeed to dislodge the cells without trypsin . Does anyone use this cell line and would be gratefull to share its knowledge.
Thank you


< Message edited by Xhulhoven -- 14/3/2006 9:15:08 AM >

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  Date 14/3/2006 9:12:14 AM

Isobel

 

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RE: Vero-Hektor  (in reply to Xhulhoven) 
Dear Xhulhoven,

EDTA alone rather than Trypsin/EDTA has been used to dislodge the Vero-Hektor cells because the Hektor G medium will not inactivate the trypsin as it is protein- and serum-free. However, if you wanted to use trypsin when harvesting these cells you would need to use a trypsin inhibitor to inactivate the trypsin.

Best wishes,

Isobel
  Date 23/3/2006 4:15:07 PM
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