How soon after blood collection do you need the samples?
Within 48 hours if at all possile.  For EDTA or Lithium Heparin tubes deterioration begins after 24 hours.  ACD tubes keep blood fresh for longer, but the lymphocyte yield will be lower.

How much blood do you need?
7 or 10ml should give us enough cells for two attempts at transformation.  But we will accept whatever is available as we understand it may sometimes be difficult to obtain a sample.

How long after a blood being delivered would the cell line be available?
6 - 8 weeks after receipt.  But we would know within 2 weeks if a sample was going to fail.