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Hybridoma Collection

ECACC No.

02043018

Cell Line Name

1D10(ATT) Clone A6

Keywords

Anti- alpha1-antitrypsin (also known as alpha1-AT

Myeloma

Sp2/0

Immunological Donor

BALB/c mouse spleen

Immunogen

Various peptides between 9 and 11 amino acids attached to PPD via a terminal cysteine residue

Sub Culture Routine

Split cells 1:2, an approximate seeding density of 5 x 10,000 cells/cm2; 5% CO2; 37°C. Please note: cells are loosely adherent, knock the flask to release attached cells. Treat as an attached cell line.

Culture Medium

RPMI 1640 + 2mM glutamine + 15% Foetal Bovine Serum (FBS)

Cell Line Description

The antibody is directed against alpha1-antitrypsin (also known as alpha1-AT or AAT).

Products

IgG1

Reactivity

Anti- alpha1-antitrypsin (also known as alpha1-AT or AAT)

Depositor

Dr. Koen Verhoef, MRC Technology, 20 Park Crescent, London, UK

Originator

No

Country

UK

References

None specified by depositor

Research Council Deposit

No

Release Conditions

No


The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer.

Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.

 

Delivery State

Price Code - C

Frozen -

 £295.00

 

Growing -

 £365.00

 

DNA -

 See Price List and manual Order Form.

 

 

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Net Total

    

    

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£295.00