General Cell Collection
ECACC No.
06041203
Cell Line Name
LA1-55n
Keywords
Human Neuroblastoma
Cell Line Description
Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma.
Species
Human
Tissue
Neural
Morphology
Fusiform cells
Passage Number
137
Sub Culture Routine
For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 to 1:100 ratio; 8% CO2, 37°C. Cells are moderate to weakly adherent. Allow floating clumps to settle then withdraw medium. Remove attached cells from substrate with 0.1% trypsin in calcium and magnesium ion free PBS or with PBS alone. Cells will detach in <5 minutes. When seeded into new flasks cells reattach readily. The population doubling time is approximately 2 days; saturation density is >1,000,000 cells/cm2. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes. Cultures grow to high density as multilayers with numerous cell clumps, which may detach and float.This cell line shows little or no transdifferentiation to the S phenotype. Cells will detach in <5 minutes. When seeded into new flasks cells reattach readily. The population doubling time is approximately 2 days; saturation density if >1000,000 cells/cm2. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes. Cultures grow to high density as multilayers with numerous cell clumps, which may detach and float.This cell line shows little or no transdifferentiation to the S phenotype.
Culture Medium
EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Karyotype
Not Specified
Depositor
Dr Robert A Ross, Laboratory of Neurobiology, Department of Biological Sciences, Fordham University, New York, USA
Originator
No
Country
USA
References
Ciccarone, V et al., (1989) Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages. Cancer Res 49, 219-25.
Additional Literature Report
Not Available
Additional Bibliography
Spengler et al., (1997) Cell lineage and differentiation state are primary determinants of MYCN gene expression and malignant potential in human neuroblastoma cells. Oncol Res 9, 467-76.
Research Council Deposit
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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