General Cell Collection
ECACC No.
06041201
Cell Line Name
LA-N-1
Keywords
Human Neuroblastoma Bone Marrow Metastasis
Cell Line Description
Established by Seeger et al., (1977) from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. The cells are neuroblastic, tear-drop shaped with multiple short fine cell processes (neurites). The cells are tumourigenic in nude mice. This is a catecholamine producing neuroblastoma cell line.
Species
Human
Tissue
Neuroblastoma
Morphology
Tear-drop shaped
Passage Number
34
Sub Culture Routine
For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 - 1:100 ratio; 8% CO2; 37°C. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Population doubling time is approximately 2 days; saturation density is >1,000,000 cells/cm2. Cells grow in weakly adherent clusters. Allow all floating clumps to settle and withdraw most of the medium. Remove attached cells from substrate with 0.1% trypsin or PBS alone, cells will detach in <5 minutes. Remaining cells, if any, are large, flat and tightly adherent precursors of Schwann cell/glia/melanocyte lineages. If desired they should be removed from the substrate with trypsin/EDTA. When the neuronal cells are seeded into a new flask the cells attach slowly and may remain in suspension for one to several days; do not change the medium the day after passage. Floating clumps of cells are viable. This passage schedule will select for cells retaining a neuroblastic phenotype; cultures transferred more frequently, with larger inocula, and/or with trypsin/EDTA may gradually lose their neuroblastic properties due to overgrowth by spontaneously arising, non-neuronal adherent cell variants. When cells are resuscitated from a frozen ampoule the cells may appear dead after a day, but reattach and resume growth within 2-3 days.
Culture Medium
EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Karyotype
Modal no. 87, range 47 - 87
Products
Catecholamine
Depositor
Dr Robert A Ross, Laboratory of Neurobiology, Department of Biological Sciences, Fordham University, New York, USA
Originator
No
Country
USA
References
Seeger et al., (1977) Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res 37, 1364-71.
Additional Literature Report
Not Available
Additional Bibliography
Ross et al., (1981) Neurotransmitter-synthesizing enzymes in 14 human neuroblastoma cell lines. Cell Mol Neurobiol 1, 301-11.
Research Council Deposit
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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