General Cell Collection
ECACC No.
06020201
Cell Line Name
VCaP
Keywords
Human Prostate Cancer Metastasis
Cell Line Description
This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.
Species
Human
Tissue
Prostate metastasis
Morphology
Epithelial
Passage Number
55
Sub Culture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Karyotype
Refer to reference (Korenchuk et al., 2001)
Receptors
Androgen receptor
Depositor
Professor Kenneth Pienta, Departments of Urology and Internal Medicine, University fo Michigan Medical Center, The University of Michigan, Ann Arbor, Michigan, USA
Originator
Yes
Country
USA
References
Korenchuk et al., (2001) VCaP, a cell-based model system of human prostate cancer. In Vivo 15, 163-8.
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
No
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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