General Cell Collection
ECACC No.
05121905
Cell Line Name
EasyCeL-Hep2
Keywords
EasyCeL Tube Format: Human negroid cervix carcinoma t
Cell Line Description
Growing Hep2 cells are supplied in cell culture tubes ready for use in clinical virology for the detection and isolation of viruses in clinical samples. Contact ECACC for more information about the EasyCeL format. Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa.
Species
Human
Tissue
Cervix
Morphology
Epithelial
Sub Culture Routine
Cell lines supplied growing in tubes are designed for single use only. Subculture is not required. On arrival incubate tubes overnight at 37C; 5%CO2. Examine cells microscopically for attachment before use.
Culture Medium
Transport medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Foetal Bovine Serum (FBS). + 100units/ml Penicillin + 0.1mg/ml Streptomycin + 2.5ug/ml Amphotericin.
Karyotype
Modal no. 14
Depositor
Obtained from ATCC
Originator
No
Country
USA
References
Cancer Res 1954;14:660; Cancer Res 1955;15:598; Soc Exp Biol Med 1956;193:107
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Tubes -
£1.30
Quantity Required
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