General Cell Collection
ECACC No.
05121902
Cell Line Name
EasyCeL-PLC/PRF/5
Keywords
EasyCeL Tube Format: Human liver hepatoma, Alexander cell line
Cell Line Description
Growing PLC/PRF/5 cells are supplied in cell culture tubes ready for use in clinical virology for the detection and isolation of viruses in clinical samples. Contact ECACC for more information about the EasyCeL format. Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.
Species
Human
Tissue
Liver
Morphology
Epithelial
Sub Culture Routine
Cell lines supplied growing in tubes are designed for single use only. Subculture is not required. On arrival incubate tubes overnight at 37C; 5%CO2. Examine cells microscopically for attachment before use.
Culture Medium
Transport medium: DMEM + 2mM Glutamine + 1% Foetal Bovine Serum (FBS) + 100units/ml Penicillin + 0.1mg/ml Streptomycin + 2.5ug/ml Amphotericin.
Karyotype
Not specified
Products
Hepatitis B Surface Antigen (HBSAg)
Depositor
Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford
Originator
No
Country
UK
References
Proc Natl Acad Sci, USA 1985;82:83; J Virol 1979;32:796; Science 1980;209:497; Science 1983;222:385; Nature 1979;282:615
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Tubes -
£1.30
Quantity Required
Prices & Ordering
Click the 'UPDATE' button to refresh the price for the quantity required.Click the Basket button to add this product to your order.
Net Total
