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General Cell Collection

ECACC No.

05121902

Cell Line Name

EasyCeL-PLC/PRF/5

Keywords

EasyCeL Tube Format: Human liver hepatoma, Alexander cell line

Cell Line Description

Growing PLC/PRF/5 cells are supplied in cell culture tubes ready for use in clinical virology for the detection and isolation of viruses in clinical samples. Contact ECACC for more information about the EasyCeL format. Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.

Species

Human

Tissue

Liver

Morphology

Epithelial

Sub Culture Routine

Cell lines supplied growing in tubes are designed for single use only. Subculture is not required. On arrival incubate tubes overnight at 37C; 5%CO2. Examine cells microscopically for attachment before use.

Culture Medium

Transport medium: DMEM + 2mM Glutamine + 1% Foetal Bovine Serum (FBS) + 100units/ml Penicillin + 0.1mg/ml Streptomycin + 2.5ug/ml Amphotericin.

Karyotype

Not specified

Products

Hepatitis B Surface Antigen (HBSAg)

Depositor

Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford

Originator

No

Country

UK

References

Proc Natl Acad Sci, USA 1985;82:83; J Virol 1979;32:796; Science 1980;209:497; Science 1983;222:385; Nature 1979;282:615

Additional Literature Report

Not Available

Additional Bibliography

Not Available

Research Council Deposit

No

Release Conditions

No

DNA Available from Stock

No


The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer.

Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.

 

Delivery State

Price Code - T

Tubes -

 £1.30

 

 

Quantity Required 

 

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Net Total

    

    

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£1.30