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General Cell Collection

ECACC No.

05071502

Cell Line Name

MDCK-SIAT1

Keywords

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Cell Line Description

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold–higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-alpha2,6-galactose moieties.

Species

Dog

Tissue

Kidney

Morphology

Epithelial

Passage Number

20

Sub Culture Routine

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO2.

Culture Medium

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Karyotype

Not specified

Receptors

Enhanced expression of 6-linked sialic acids

Depositor

Mikhail Matrosovich, Institute of Virology, Philipps University, Marburg, Germany. E-mail: [email protected]

Originator

Yes

Country

Germany

References

Matrosovich M, Matrosovich T, Carr J, Roberts NA & Klenk H. (2003) Overexpression of the alpha-2,6-sialyltransferase in MDCK cells increases influenza virus sensitivity to neuraminidase inhibitors. J. Virol. 77:8418-8425

Additional Literature Report

Not Available

Additional Bibliography

Not Available

Research Council Deposit

No

Release Conditions

No

DNA Available from Stock

No


The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer.

Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.

 

Delivery State

Price Code - C

Frozen -

 £295.00

 

Growing -

 £365.00

 

DNA -

 See Price List and manual Order Form.

 

 

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Net Total

    

    

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£295.00