General Cell Collection
ECACC No.
05071502
Cell Line Name
MDCK-SIAT1
Keywords
Canine Cocker Spaniel Kidney Sialic Acid Over Expression
Cell Line Description
This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold–higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-alpha2,6-galactose moieties.
Species
Dog
Tissue
Kidney
Morphology
Epithelial
Passage Number
20
Sub Culture Routine
Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Culture Medium
DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).
Karyotype
Not specified
Receptors
Enhanced expression of 6-linked sialic acids
Depositor
Mikhail Matrosovich, Institute of Virology, Philipps University, Marburg, Germany. E-mail: [email protected]
Originator
Yes
Country
Germany
References
Matrosovich M, Matrosovich T, Carr J, Roberts NA & Klenk H. (2003) Overexpression of the alpha-2,6-sialyltransferase in MDCK cells increases influenza virus sensitivity to neuraminidase inhibitors. J. Virol. 77:8418-8425
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
No
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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