General Cell Collection
ECACC No.
04072120
Cell Line Name
293TGPRT+R1-A
Keywords
Packaging cell line for HIV based vectors
Cell Line Description
293T cells serially transfected with wild-type HIV Gag-Pol sequences expressed from a Murine Leukaemia Virus (MLV) vector; HIV tat sequences expressed from a MLV vector; HIV rev sequence expressed from a MLV vector, extra wild-type HIV-rev expressed from a MLV vector and amphotropic envelope protein expressed from a MLV vector. These cells stably express wild-type HIV Gag-Pol, HIV tat and HIV rev sequences, as well as extra wild-type HIV rev and amphotropic envelope protein. These cells serve as a packaging cell line for HIV based vectors.
Species
Human
Tissue
Kidney, embryo
Morphology
Epithelial
Passage Number
+4
Sub Culture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells may take up to 7 days to recover after resuscitation. If very few cells survive after 48 hours, it is advisable to wait another 2-7 days. During this time changing the media should be avoided as cells that are less than 10% confluent are extremely sensitive to any form of shock. These cells are more sensitive to pH and temperature shock than normal 293T cells, it is advisable to pre-warm media at 37oC before use. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. These cells must be frozen in glycerol not DMSO.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype
Not specified
Depositor
Professor Mary Collins, Department of Immunology and Molecular Pathology, University College London
Originator
Yes
Country
UK
References
Ikeda Y et. al., Nat. Biotechnol. (2003) May: 21(5): 569-572
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
No
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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