General Cell Collection
ECACC No.
05063001
Cell Line Name
COS-7-InVitrus
Keywords
Monkey African green kidney, SV40 transformed, serum-free
Cell Line Description
COS-7 cell line (ECACC catalogue no. 87021302) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent i.e. adherent. COS-7 was derived from CV-1 simian cells transformed by an origin-defective mutant of SV40.
Species
Monkey
Tissue
Kidney
Morphology
Fibroblast
Passage Number
+5
Sub Culture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Culture Medium
InVitrus medium (Cell Culture Technologies) + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO.
Karyotype
Not Specified
Depositor
Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury, SP4 0JG, UK
Originator
Yes
Country
UK
References
None Specified By Depositor
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
No
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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