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General Cell Collection

ECACC No.

05062302

Cell Line Name

BHK21-InVitrus

Keywords

Hamster Syrian kidney, serum-free

Cell Line Description

BHK21CL13-2P cell line (ECACC catalogue no. 84111301) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies).

Species

Hamster

Tissue

Kidney

Morphology

Fibroblast

Passage Number

+13

Sub Culture Routine

Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO2; 37°C. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Culture Medium

InVitrus medium (Cell Culture Technologies) + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use InVitrus medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.

Karyotype

Not Specified

Depositor

Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland.

Originator

Yes

Country

Switzerland

References

None Specified By Depositor

Additional Literature Report

Not Available

Additional Bibliography

Not Available

Research Council Deposit

No

Release Conditions

No

DNA Available from Stock

No


The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer.

Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.

 

Delivery State

Price Code - C

Frozen -

 £295.00

 

Growing -

 £365.00

 

DNA -

 See Price List and manual Order Form.

 

 

Quantity Required 

 

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Net Total

    

    

Print

£295.00

 

 

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