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General Cell Collection

ECACC No.

05030204

Cell Line Name

293-Hektor

Keywords

Human Embryo Kidney, serum-free

Cell Line Description

The human embryo kidney cell line 293 (ECACC catalogue no. 85120602) adapted to grow in the chemically defined protein- and peptide-free Hektor G medium (Cell Culture Technologies). Cells grow in suspension and tend to form aggregates in static culture. The cells grow as finely dispersed single cell suspension under optimal agitated culture conditions.

Species

Human

Tissue

Kidney

Morphology

Fibroblast

Passage Number

+20

Sub Culture Routine

Use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C. Resuscitate contents of ampoule into a total of 10ml medium in a T25 flask. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

Culture Medium

Hektor G medium (Cell Culture Technologies) + 4mM Glutamine + 5 ug/ml Insulin. The exogeneous insulin supplementation can be reduced or totally eliminated under optimal culture conditions. When freezing cells down use culture medium (50:50 conditioned me

Karyotype

Not Specified

Depositor

Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland.

Originator

Yes

Country

Switzerland

References

None Specified By Depositor

Additional Literature Report

Not Available

Additional Bibliography

Not Available

Research Council Deposit

No

Release Conditions

No

DNA Available from Stock

No


The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer.

Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.

 

Delivery State

Price Code - C

Frozen -

 £295.00

 

Growing -

 £365.00

 

DNA -

 See Price List and manual Order Form.

 

 

Quantity Required 

 

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Net Total

    

    

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£295.00