General Cell Collection ECACC No. 05030204 Cell Line Name 293-Hektor Keywords Human Embryo Kidney, serum-free Cell Line Description The human embryo kidney cell line 293 (ECACC catalogue no. 85120602) adapted to grow in the chemically defined protein- and peptide-free Hektor G medium (Cell Culture Technologies). Cells grow in suspension and tend to form aggregates in static culture. The cells grow as finely dispersed single cell suspension under optimal agitated culture conditions. Species Human Tissue Kidney Morphology Fibroblast Passage Number +20 Sub Culture Routine Use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37�C. Resuscitate contents of ampoule into a total of 10ml medium in a T25 flask. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO. Culture Medium Hektor G medium (Cell Culture Technologies) + 4mM Glutamine + 5 ug/ml Insulin. The exogeneous insulin supplementation can be reduced or totally eliminated under optimal culture conditions. When freezing cells down use culture medium (50:50 conditioned me Karyotype Not Specified Depositor Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland. Originator Yes Country Switzerland References None Specified By Depositor Additional Literature Report Not Available Additional Bibliography Not Available Research Council Deposit No Release Conditions No DNA Available from Stock No The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.   Delivery State Price Code - C Frozen -  �295.00   Growing -  �365.00   DNA -  See Price List and manual Order Form.     Quantity Required    Prices & Ordering Click the 'UPDATE' button to refresh the price for the quantity of packs required. Click the Basket button to add this product to your order.    Net Total           �295.00