General Cell Collection
ECACC No.
04072106
Cell Line Name
BICR 22
Keywords
Tongue squamous carcinoma (lymph node metastasis)
Cell Line Description
Adherent cell line derived from a lymph node metastasis squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.
Species
Human
Tissue
Tongue
Morphology
Epithelial, keratinocyte
Passage Number
9
Sub Culture Routine
Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37°C. Suggested seeding density 2 x 10,000 cells/cm2. Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells.
Culture Medium
DMEM + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Karyotype
Aneuploid
Receptors
Epidermal Growth Factor Receptor (EGFR)
Depositor
Dr. Kenneth Parkinson, Cancer Research Campaign Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD
Originator
Yes
Country
UK
References
Edington KG et.al. Mol.Carcinogenesis 13:254-265 (1995); Loughran O et.al. April 24: Oncogene 14 (16):1955-1964 (1997); Munro J et.al. Cancer Research, 59: 2516-2521,(1999).
Additional Literature Report
Not Available
Additional Bibliography
Research Council Deposit
No
Release Conditions
DNA Available from Stock
The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer. Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
Delivery State
Frozen -
£295.00
Growing -
£365.00
DNA -
See Price List and manual Order Form.
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