Sub Culture Routine
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Split semi-confluent cultures 1:3 i.e. seeding at 4x10,000 cells/cm� using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37�C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. In order to prevent syncitia formation, do not allow cells to become confluent.
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Depositor
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Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland.
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The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details
distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where
quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore
further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and
ECACC does not guarantee the passage number stated will be the passage number received by the customer.
Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.
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