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General Cell Collection

ECACC No.

03092501

Cell Line Name

Sp2/0-Ag14-TurboDoma

Keywords

Mouse x Mouse hybridoma, non-producing, serum-free

Cell Line Description

Sp2/0-Ag14 cell line (ECACC catalogue no. 85072401) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma (Cell Culture Technologies). Suitable for use as a fusion parent for serum-free generation of hybridomas as well as a host for the production of recombinant proteins.

Species

Mouse

Tissue

Unknown

Morphology

Lymphoblast

Sub Culture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Culture Medium

TurboDoma medium (Cell Culture Technologies) + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

Karyotype

Not Specified

Depositor

Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies Inc., Via al Chioso 14, 6929 Gravesano, Switzerland, Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland, and Ing. Claudio Strebel of CePower Inc., Einsiedlerstr. 29, 8820 Waedenswil, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland.

Originator

Yes

Country

Switzerland

References

None Specified By Depositor

Additional Literature Report

Not Available

Additional Bibliography

Not Available

Research Council Deposit

No

Release Conditions

No

DNA Available from Stock

No


The ECACC collections represent deposits of cell cultures from world-wide sources. While every effort is made to ensure details distributed by ECACC are accurate, ECACC cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and ECACC does not guarantee the passage number stated will be the passage number received by the customer.

Cell lines supplied by ECACC are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the ECACC Terms & Conditions of Supply for more information.

 

Delivery State

Price Code - C

Frozen -

 £295.00

 

Growing -

 £365.00

 

DNA -

 See Price List and manual Order Form.

 

 

Quantity Required 

 

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Net Total

    

    

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£295.00